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. 2020 Jul 30;9(8):1807. doi: 10.3390/cells9081807

Figure 2.

Figure 2

Fyn triggered APP phosphorylation at Tyr682 in human neurons. (A) Untransfected control neurons (Ctrl) and APP, APP+Fyn, APP+dnFyn, and APP YG+Fyn were immunoprecipitated with mouse anti-pTyr magnetic beads and analyzed by WB with rabbit anti-APP antibody. WB densitometric analysis is reported in (B). Values were calculated by dividing pAPP-Tyr by the corresponding APP optical density values (pAPP-Tyr/APP). Statistically significant differences were calculated using one-way ANOVA followed by Dunnett’s post hoc test. n = 3. * p ≤ 0.05, vs. APP; ** p < 0.05, vs. Fyn. (C) WB analysis of APP, Fyn, pFyn-Tyr420, and β-actin from total lysates of untransfected control neurons (Ctrl) and APP, APP+Fyn, APP+dnFyn, and APP YG+Fyn. pFyn-Tyr420 levels were calculated as a ratio of pFyn-Tyr420 relative to the corresponding Fyn optical density values (pFyn-Tyr420/Fyn). The densitometric analysis is reported in (D). The data are expressed as a percentage of untransfected control (empty vector). Statistically significant differences were calculated using one-way ANOVA followed by Dunnett’s post hoc test. n = 3. * p < 0.05, vs. control (black bar).