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. 2020 Jul 29;12(8):2101. doi: 10.3390/cancers12082101

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Estimated levels of amylase-sensitive glycogen with regard to the monosomy-3 status and nuclear BAP1 expression. The difference in the intensity of the periodic acid-Schiff (PAS) stainings (A) without and (B) with amylase pretreatment was taken as the amount of amylase-sensitive glycogen. The amylase pretreatment has also led to a weakening in the staining of the extracellular arcs (black arrows). Original magnification: 100×. (C) Expression of BAP1 was analyzed by immunohistochemistry and staining of the nuclei with nuclear fast red. Original magnification: 200×. (D) The copy number of chromosome-3 (red) together with the melanoma marker protein Melan-A (green) was determined by performing a combined immunostaining with fluorescent in situ hybridization (Immuno-FISH) on the paraffin sections. Nuclei were counterstained in blue with 4′,6-diamidino-2-phenylindole. Arrows indicate several cells with monosomy-3. Scale bar = 10 µm.