Table 2.
Haplotypes detected based on fragment length polymorphism using six chloroplast microsatellites (cpSSR) and three plastid DNA regions in the germplasm belonging to the studied manna ash collection. F. ornus was used as reference. In bracket the number of samples analyzed belonging to each local variety/species.
| Samples/Local Varieties | Nh # | ccmp2 | ccmp3 | ccmp4 | ccmp6 | ccmp7 | ccmp10 | CPFRAX6 | atpB/rbcL | matK | Haplotypes * |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Abbassa cappeddu (3) | 1 | 194 | 97 | 140 | 98 | 117 | 104 | 365 | 157 | 253 | H10 |
| Baciciu (4) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Cavolo (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Macigna (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Nivuru (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Nsiriddu (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 366 | 157 | 253 | H5 a |
| Russu (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Sarvaggio (3) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Verdello (7) | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 | |
| 3 | 194 | 97 | 140 | 98 | 117 | 103 | 366 | 157 | 253 | H5 a | |
| 194 | 97 | 140 | 98 | 117 | 104 | 365 | 157 | 253 | H10 | ||
| Frassino monumentale (1) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| Nsiriddu monumentale (1) | 1 | 194 | 97 | 140 | 98 | 117 | 103 | 365 | 157 | 253 | H5 |
| F. ornus (4) | 1 | 190 | 97 | 140 | 99 | 117 | 106 | 364 | 157 | 253 | H19 |
# Nh, number of haplotypes. * Haplotype classification following Heuertz et al. (2006) [4], without CPFRAX6, atpB/rbcL and matK profiles.a H5 sub-variation identified using also CPFRAX6, atpB/rbcL and matK profiles.