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. 2020 Aug 7;10(8):1160. doi: 10.3390/biom10081160

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Visual representation of the discussed antisense probe-based methods. Details of following techniques can be find in Section 3.2: (A) Peptide nucleic acid (PNA)-assisted identification of RBPs (PAIR), (B) Capture hybridization analysis of RNA targets (CHART), (C) Chromatin isolation by RNA purification (ChIRP), (D) RNA antisense purification (RAP), (E) Specific RNP capture with antisense LNA (locked nucleic acid)/DNA mixmers, (F) Hybridization purification of RNA-protein complexes (HyPR-MS), (G) pentatricopeptide repeat proteins (PPR), (H) Biotin-tagged Cas9 purification.