Figure 1.

Y-box binding protein-1 (YB-1) is essential for tumor necrosis factor (TNF)-induced NF-κB nuclear translocation in bone marrow-derived macrophages. (A) Imaging flow cytometry showing one representative cell. The nucleus stained positive for 7-Amino-actinomycin D (7AAD) (red). Staining for NF-κB phospho-p65 activation (pp65) is shown with and without stimulation (green). Colocalization (yellow) indicates nuclear translocation. (B) Normalized frequency of wild type (WT) (black) and YB-1 knockout (KO) (Ybx1^ΔLysM) cells (red) showing nuclear translocation of activated NF-κB p65 with and without TNF stimulation (20 ng/mL).