Figure 3.

YB-1 was protective against cell death in vitro. (A,B) Representative flow cytometry dot plot analyses of THP-1 (A) and U937 cells (B). Cells were treated with or without TNF (20 ng/mL) and cycloheximide (10 ng/mL) alone or together overnight. Cells were harvested and stained for annexin V and 7AAD. (C) Graphs representing flow cytometry analysis of live and dead cells in THP-1 and U937 cells. Statistical significance was calculated by two-way ANOVA with Bonferroni post hoc test, n = 3. Data represent the mean ± SEM. ** p < 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.