Figure 6.

Cell death was rescued using the pan-caspase inhibitor zVAD-fmk (ZVAD). (A) THP-1 cells were pretreated with zVAD and/or necrostatin-1 (Nec-1) for 1 h and treated with TNF and CHX overnight. The cells were harvested, stained with propidium iodide (PI), and analyzed by flow cytometry. The percentage of dead cells following treatment is indicated. (B) Graphs representing flow cytometry analysis of THP-1 (left panel) and U937 (right panel). Error bars specify the standard error mean (SEM) of at least three experiments. Statistical significance was calculated by two-way ANOVA with Bonferroni post hoc test, n = 3. Data represent the mean ± SEM. *** p ≤ 0.001, **** p ≤ 0.0001.