Table 3.
Antioxidant activities of standards and the methanol extracts from different parts of A. macrocephalus subsp. finitimus x.
| Assays | Aerial Parts | Flowers | Leaves | Roots | Stems | Trolox | EDTA |
|---|---|---|---|---|---|---|---|
| Effective concentration (EC50: mg/mL) | |||||||
| Phosphomolybdenum | 2.52 ± 0.23 c | 2.67 ± 0.08c | 1.81 ± 0.12 b | 2.77 ± 0.03 c | 2.77 ± 0.15 c | 1.14 ± 0.02 a | - |
| DPPH radical | 9.30 ± 0.20 c | 14.20 ± 0.12e | 2.65 ± 0.05 b | 10.54 ± 0.52 d | 8.73 ± 0.22 c | 0.26 ± 0.02 a | - |
| ABTS radical cation | 1.66 ± 0.01 b | 3.35 ± 0.01d | 1.45 ± 0.04 b | 3.88 ± 0.11 e | 1.93 ± 0.07 c | 0.25 ± 0.02 a | - |
| CUPRAC reducing power | 2.37 ± 0.06 c | 3.62 ± 0.16d | 1.06 ± 0.02 b | 5.28 ± 0.39 e | 2.90 ± 0.12 cd | 0.32 ± 0.03 a | - |
| FRAP reducing power | 1.75 ± 0.02 c | 2.46 ± 0.05d | 0.73 ± 0.01 b | 2.83 ± 0.12 e | 1.93 ± 0.08 c | 0.12 ± 0.02a | - |
| Ferrous ion chelating | 1.08 ± 0.01 b | 1.03 ± 0.01b | 1.65 ± 0.05 c | 1.07 ± 0.01 b | 1.05 ± 0.03 b | - | 0.036 ± 0.004 a |
| Antioxidant activity | |||||||
| Phosphomolybdenum (mmol TEs/g extract) | 1.85 ± 0.17 b | 1.73 ± 0.05b | 2.56 ± 0.17 a | 1.67 ± 0.02 b | 1.67 ± 0.09 b | - | - |
| DPPH radical (mg TEs/g extract) | 24.97 ± 0.61 bc | 15.42 ± 0.15d | 94.66 ± 1.97 a | 21.75 ± 1.21 c | 26.80 ± 0.76 b | - | - |
| ABTS radical cation (mg TEs/g extract) | 161.59 ± 0.86 b | 78.94 ± 0.01d | 185.65 ± 5.03 a | 67.98 ± 2.01 d | 138.54 ± 4.74 c | - | - |
| CUPRAC reducing power (mg TEs/g extract) | 126.91 ± 3.50 b | 78.76 ± 3.96d | 297.74 ± 4.90 a | 50.23 ± 4.64 e | 101.35 ± 4.66 c | - | - |
| FRAP reducing power (mg TEs/g extract) | 59.81 ± 0.67 b | 42.59 ± 0.89c | 142.69 ± 2.15 a | 37.05 ± 1.63 c | 54.37 ± 2.15 b | - | - |
| Ferrous ion chelating (mg EDTAEs/g extract) | 66.81 ± 0.36 a | 70.11 ± 0.51a | 43.41 ± 1.39 b | 67.45 ± 0.18 a | 68.59 ± 2.00 a | - | - |
x Within each row, means sharing the different superscripts show comparison between the samples using Tukey’s test at p < 0.05. EC50 (mg/mL), effective concentration at which the absorbance was 0.5 for reducing power and phosphomolybdenum assays and at which 50% of the DPPH and ABTS radicals were scavenged and the ferrous ion-ferrozine complex were inhibited. EDTA, ethylenediaminetetraacetic acid (disodium salt). “-”, not determined. TEs and EDTAEs, trolox and ethylenediaminetetraacetic acid (disodium salt) equivalents, respectively.