Figure 2.

The effect of metformin (Met) and everolimus (Eve) on QGP-1 and H727 cell viability. (A,B) Cells were incubated with metformin (50 µM, 100 µM and 400 µM), or treated with everolimus (10 nM), or with the combination of everolimus 10 nM and metformin (50 µM, 100 µM and 400 µM) for 7 days. The combination of metformin 400 µM and everolimus 10 nM induced a more effective reduction of both cell lines’ viability than each monotherapy. Values represent mean (± SD.) * = p < 0.05, **** = p < 0.0001 vs. corresponding basal, # = p < 0.05 and #### = p < 0.0001 vs. everolimus, ° = p < 0.05 and °° = p < 0.01 vs. metformin. Experiments were repeated at least 3 times and each determination was done in triplicate. Statistical analysis was performed with a one-way ANOVA test followed by Tukey’s post-hoc test.