Table 2.
Composition and responses of the Box–Behnken factorial design.
| Standard Run. | Independent Variables | Z-Average * (nm) | PdI * | ||
|---|---|---|---|---|---|
| Soluplus® (mg/mL) | Ethanol (mL) | 1-M NaOH (mL) | |||
| 1 | 6.0 | 5.0 | 4.5 | 194.5 ± 1.8 | 0.411 ± 0.02 |
| 2 | 12.0 | 5.0 | 4.5 | 188.0 ± 3.9 | 0.299 ± 0.09 |
| 3 | 6.0 | 10.0 | 4.5 | 94.87 ± 1.1 | 0.155 ± 0.02 |
| 4 | 12.0 | 10.0 | 4.5 | 270.1 ± 2.0 | 0.513 ± 0.07 |
| 5 | 6.0 | 7.5 | 3.0 | 105.6 ± 0.9 | 0.164 ± 0.01 |
| 6 | 12.0 | 7.5 | 3.0 | 170.4 ± 5.6 | 0.477 ± 0.04 |
| 7 | 6.0 | 7.5 | 6.0 | 116.2 ± 1.5 | 0.140 ± 0.03 |
| 8 | 12.0 | 7.5 | 6.0 | 200.12 ± 7.3 | 0.344 ± 0.11 |
| 9 | 9.0 | 5.0 | 3.0 | 195.0 ± 2.3 | 0.377 ± 0.05 |
| 10 | 9.0 | 10.0 | 3.0 | 111.6 ± 3.0 | 0.114 ± 0.06 |
| 11 | 9.0 | 5.0 | 6.0 | 65.4 ± 1.6 | 0.401 ± 0.17 |
| 12 | 9.0 | 10.0 | 6.0 | 99.5 ± 1.7 | 0.444 ± 0.06 |
| 13 | 9.0 | 7.5 | 4.5 | 99.86 ± 0.5 | 0.134 ± 0.04 |
| 14 | 9.0 | 7.5 | 4.5 | 100.12 ± 0.7 | 0.155 ± 0.08 |
| 15 | 9.0 | 7.5 | 4.5 | 107.7 ± 3.1 | 0.163 ± 0.05 |
* Data are means ± SD (n = 3 independent formulations).