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. 2020 Aug 13;10(8):548. doi: 10.3390/brainsci10080548

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of 2-CdA treatment on Smac/DIABLO and Bcl-2 protein expression in SH-SY5Y single-cultured and co-cultured with HD and MS lymphocytes. (A) Representative Western blot of Smac/DIABLO and Bcl-2 levels performed on whole cell lysates. Bar graphs show quantification by densitometric analysis of Smac/DIABLO (B) and Bcl-2 (C) protein band normalized to GAPDH, used as loading control. Values (mean ± SEM of three independent experiments) are expressed as percentage of dimethyl sulfoxide (DMSO). Significance was calculated with Student’s t-test vs. DMSO.