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. 2020 Aug 13;10(8):548. doi: 10.3390/brainsci10080548

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative dot plots of annexin V/SYTOX Green assay on SH-SY5Y cells exposed to 20 nM 2-CdA for 4, 24, 48, and 72 h. The population, gated on not treated events (control, not shown in dot plots) was divided into live events (lower left), apoptotic events (lower right), and late apoptotic events (upper right). The percentage of apoptotic events (lower right) was similar among the four time points analyzed: 12.48 ± 1.59% at 4 h; 17.68 ± 2.25% at 24 h; 15.20 ± 1.94% at 48 h; 14.45 ± 1.67% at 72 h (data are expressed in mean of percentage ± SD; n = 4).