Figure 3.

Detection of HPyV6 on the transcriptional level in FFPE CCA tissue by RNA-ISH, the CCA2 patient tissue section was hybridized with 20 sets labeled probes to detect HPyV6 LTAg mRNA using RNAscope RNA in situ hybridization assay. Additionally, FFPE hybridized with both the bacterial gene (DapB) as a negative control and the housekeeping gene (PPIB) as a positive control. Positive red signals were detected using fast red chromogen. HPyV6 LTA transcript seen as red signals in CCA and hepatocytes. No HPyV6 RNA was seen when the tissue was treated with RNAse, nuclei were counterstained with hematoxylin. The images were taken at 200x magnification, a black square area was magnified 6x in the top right corner of each figure.