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. 2020 Aug 11;12(8):756. doi: 10.3390/pharmaceutics12080756

Figure 2.

Figure 2

Cellular internalization and uptake of DiI or FAM-miR125/SLN-KL into human tongue squamous carcinoma SAS cells. (a,b) SAS cells were treated with (a–c) DiI in LPN-KL or (b–d) FAM-miR125/SLN-KL for the indicated time intervals and observed with CLSM. (a) Green, MitoGreen; red, DiI; blue, DAPI; gray, EGFR. (b) Green, FAM-miR125; red, MitoRed; blue, DAPI; gray, EGFR. (c) Green, LysoGreen; red, DiI; blue, DAPI; gray, EEA1. (d) Green, FAM-miR125; red, LysoRed; blue, DAPI; gray, EEA1. Magnification: 1500×. The representative images are shown (n = 3). DiI, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate; FAM, carboxyl fluorescein; EEA1, early endosome antigen 1.