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. 2020 Aug 1;12(8):2137. doi: 10.3390/cancers12082137

Figure 1.

Figure 1

Identification of a super-enhancer related to the MCL1 gene in glioblastoma (GBM) tissues and cell cultures. (a) Chromatin immunoprecipitation (CHIP) with an antibody binding to H3K27ac coupled with next generation sequencing was performed on GBM1 (GBM tissue) and U87 GBM cells. Following peak calling for enhancer regions, the peaks were ranked based on the Tag count (Tag count vs. Enhancer Rank; hockey stick plot). The coordinates of the super-enhancer related to the Mcl-1 gene are chromosome 1:150,601,879-150,630,909 (GRCh38/hg38). The GBM1 chromatin immunoprecipitation with next generation sequencing (CHIP-seq) data for the super-enhancer analysis were downloaded from GEO (GSE119755); (b) Shown are H3K27ac CHIP-seq plots around the MCL1 locus in GBMs and normal brain tissue. The GBM tissues and the normal brain were downloaded from GEO (GSE101148; GRCh19/hg19); (c) Shown are H3K27ac CHIP-seq plots around the MCL1 locus in established GBM cells (LN229 and U87) and in stem-like NCH644 GBM cells; (d) Shown are the mRNA levels of Mcl-1 expression in Shai brain or TCGA brain databases in glioblastomas compared to normal brain tissue from oncomine (www.oncomine.org, 05/2020, Thermo Fisher, Waltham, MA, USA) [24]; (e) Genomic regions enrichment of annotations tool (GREAT) analysis of super enhancer genes “GO biological process.”