Figure 1.

Distinguishing Epilepsy vs. Control patients using leave one [serum sample] out cross-validation/ peak classification value (LOOCV/PCV) serum mass peak analysis. (A) Flow chart outlining the serum sample handling and mass spectrometry processing of the binary patient/subject group analysis. (B) Serum mass peak Scoring of the LOOCV/PCV (leave (one serum sample) out cross-validation/peak classification value) procedure used to classify mass peaks either as “epilepsy” or as “control” for a “left out” sample, (a limited sample range 900–1008 m/z is displayed) of significant group discriminatory mass peaks. The PCV example for the peak at 919 is exhibited and used to classify “left out” peaks as either “epilepsy” (peak area above this PCV) or control (peak area at or below this PCV). (C) Serum discrimination of epilepsy patients (dashes) from controls (triangles) by a % of LOOCV classified mass peaks. A cut off value is indicated (− or + SDs from the seizure or control groups respectively) to determine test metric values (e.g., true positives). (D) A lack of serum sample discrimination is demonstrated which results when the two different sample groups are mixed together randomly followed by the same LOOCV mass peak analysis.