Strengths:

Reagents are cheap, relatively stable, and more accessible than DPPH and ABTS reagents [81] Rapid colour development [81] Can screen both lipophilic and hydrophilic samples [81] The assay is performed at pH 7, which is close to the physiological pH in contrast to the unrealistic acidic pH 3.6 of FRAP [81] Effective for evaluating the AO capacity of synthetic mixtures [81] It can detect glutathione and thiol-type AO in contrary to FRAP. The reason lies behind the fact that Fe (III) has a half-filled d orbital which contribute to its chemical inertness, while the electronic structure of Cu (II) triggers fast kinetics [87] Simple instrumentation required No interferences from chemicals found in solutions reported yet Shows good correlation with numerous polyphenolics namely flavonoids, phenolic acids and also, with other AO methods, namely ABTS A linear correlation is usually observed with the phosphomolybdenum assay

Limitations:

Unable to measure antioxidant enzymes [81] Depending on CUPRAC version, longer times of measurement may be required [81] Sometimes requires incubation at 50 °C in a water bath for 20 min for compounds which develop color slowly namely naringin and naringenin [82]