Strengths:

Cheap, simple, and reliable method [88] Since absorbance is read at a high wavelength, possible interference from the reaction mixture is minimized [90] Simple instrumentation is used

Limitations:

If the samples are very active, pellets can be observed in tubes. Thus, centrifugation will be needed The assay cannot be performed in microplates which can be time-consuming and tiring while screening many samples If samples have a high level of protein, precipitation with trichloroacetic acid may be hard