Strengths:

Formation of the phosphomolybdenum complex is independent of the different organic solvents (hexane, methanol, ethanol, and dimethyl sulfoxide) used to prepare AO or extract stock solution [95] Simple, sensitive, and cheap reagents are used [95] Can screen a wide spectrum of samples, including lipophilic plant extracts, vegetal oils, butter, serum, pharmaceutical, and cosmeceutical samples [95]

Limitations:

Bad correlation is observed with bioactive compounds (phenolics, flavonoids) [86] A weak correlation is usually reported with free radical scavenging assays, namely DPPH [99] Time-consuming. Since high temperature (95 °C) is required, the assay should be performed in test tubes instead of microplates, which could be problematic while screening a large number of samples Bad correlation with bioactive compounds in essential oil samples due to their complex chemical composition Non-specific, since the assay does not detect only phenolics but also ascorbic acid, carotenoids and α-tocopherol [95]