Strengths:

Easily available and cheap reagents [17] Simple instrumentation is used Good repeatability and reproducibility [17] Assay can be carried out in both test tubes and 96-well microplates

Limitations:

Non-specific, since this assay does not only react with phenolic compounds but also with peptides and sulphates present in the medium Sometimes, results from total bioactive components (phenolic, flavonoids) assay do not correlate with the results of metal chelating Bad correlation with FRAP, CUPRAC, ABTS, and DPPH assays