Strengths:

Highly reproducible [112] Can screen both lipophilic and hydrophilic samples [114]

Limitations:

Time-consuming Sometimes, the results from total bioactive components (phenolic, flavonoids) assay do not correlate with the results of this assay Bad correlation with numerous assays, namely FRAP, CUPRAC, ABTS, and DPPH Difficulty in interpreting results in the presence of bad correlations Low reproducibility [113] Sensitive to oxygen, pH, temperature, and solvent effects [111,112]