Strengths:

Direct physiological resemblance of peroxynitrite (ONOO-) to the human body [61]

Limitations:

Lacks specificity. The rapid decomposition of ONOO− forms NO• and O2•−. These two species have the potential to oxidize DHR 123. Thus, it can be said that the oxidation of DHR 123 is not directly linked to ONOO− [62] Require expensive equipment and reagent, namely fluorescence spectrophotometer, −80 °C freezer, and DHR 123, which are not easily accessible in all laboratories