Strengths:

Easy and sensitive [59] Specific [80] Can quantify H2O2 level in cells [78] Reagents are easily available

Limitations:

Substrates are pH sensitive [80] Several reductants/or antioxidants for e.g., thiols, ascorbate can compete with scopoletin, leading to an underestimation of H2O2 formation [80] Similarly, catalase released from disruption of cells may compete with HRP for H2O2 [80] Quartz cuvettes should be used which are expensive and not affordable by all laboratories