Figure 4.

3D reconstructions of the cartilage during the time-lapse. In column (a) the CARS signal is shown in red at 2848 cm⁻¹ originating mainly in the cells lipid structures; in column (b) the SHG signal generated by the collagen is shown in blue; in column (c) the TPEF signal related to the EVs fluorescence is shown in green. In column (d) all the three signals are visualized together, while in column (e) only the CARS and the TPEF are visualized, and in column (f) only the SHG and the TPEF are visualized. In the top right corner of each image the related timeframe is depicted and close to the boundary box the dimensions in μm are shown starting from the origin vertex.