Figure 2.

CDDP decreased the activity of the epithelial Na⁺ channel (ENaC) in native murine Principal cells. (A) Representative current traces of ENaC in cell-attached patches made on the apical plasma membrane of Principal cells in freshly isolated tubules from naïve mice before (top) and after (bottom) treatment with 1.5 µM CDDP. Negative pipette potential −60 mV and inward Na⁺ current downwards. (B) Summary graph of (n = 7) paired experiments showing ENaC activity (NP_o) before and after treatment with CDDP. Summary data from experiments were identical to that shown in (A) * p < 0.05 vs. Before.