Figure 2.

Physiologic parameters and analysis of the bronchoalveolar lavage (BAL). (A) Body weight every 24 h, considering 100% as the starting body weight for each group. (B) Survival of the animals over the timeline of the experiment. (C) Ratio of lung weight corrected by body weight measured at the end of the experiment (grams/grams; n = 6–12). (D) Amount of IgM measured by ELISA in BAL fluid at the endpoint as a representation of the permeability (n = 6–8). (E) Protein concentration in µg/mL in the BAL fluid at the endpoint (n = 6–8). (F) Percentage of neutrophils, macrophages, and lymphocytes in the recovered BAL fluid performed in the unilobilar lung at the endpoint. Lymphocytes are less than 1% in all groups and are almost not visible (n = 5–7 per group). (G) Myeloperoxidase (MPO) measured by ELISA in the BAL fluid (n = 4–6). Data are representative from 3 independent experiments (mean ± SEM; each point represents one animal). ANOVA followed by a Newman–Keuls multiple-comparison test was used to evaluate the significant differences * p < 0.01 vs. the corresponding control group (no differences among the three controls groups was observed in any parameter evaluated); # p < 0.01 vs. the HCl + LPS group.