PD-L1 ligand expression by different T cell subpopulations throughout pregnancy and in nonpregnant women. The expression of PD-L1 molecule by CD8+T cell (A), CD4+T (B), Treg (C) and NKT-like (D) subpopulations. The solid bars represent medians of 10, 13, 10 and 12 samples, the boxes indicate the interquartile ranges, and the lines show the most extreme observations. Differences were considered statistically significant for p-values ≤ 0.05. Variables with the same letter indicate no statistically significant difference, while different letters indicate a statistically significant difference.