Figure 4.

Characterization of apoptosis induced by marine-derived compounds with and without TRAIL in Jurkat and HCT116 cells. (A,B) Cells were treated with 50 µM of the indicated compounds for 6 h in the absence and in the presence of TRAIL (250 ng) and stained with the fluorescent DNA-binding dye Hoechst 33342. Morphological changes (A,B) were detected under fluorescent microscope using a blue filter. Corresponding quantifications are shown in (C,D). All values are presented here as ±SD (n = 3). Significance was evaluated by two-way ANOVA. **** p < 0.0001.