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. 2020 Aug 5;12(8):2189. doi: 10.3390/cancers12082189

Figure 6.

Figure 6

Sonicated hyaluronan (HAson) blocks melanoma and breast cancer growth in mice. (A) BALB/c mice pretreated with antibody against Hyal-2 (10 μL serum in 90 μL PBS), 200 μg native HA (HAn in 100 μL PBS), or 200 μg modified HA (sonicated HAson or UV irradiated HAuv) via tail vein injections for three consecutive days. 20 days later, mice were subcutaneously inoculated with B16F10 cells. (B) Under similar conditions, BALB/c mice received tail vein injections with hyaluronan preparations for three consecutive days, followed by inoculation with syngeneic 4T1 breast cancer cells. Statistical analysis for B: * p < 0.05, n = 4, Student’s t test (all groups versus PBS group). (C) Similarly, both Hyal-2 antibody and HAson suppressed B16F10 growth in NOD-SCID mice. (D) Both sonication and UV irradiation caused HA degradation, as compared to the native HA. (E) Similarly, HAson6 blocked breast MDA-MB-231 cell growth in NOD-SCID mice. HAn = native HA; HAson3 or 6 = HAn sonicated for three and six hours, respectively. NRS = normal rabbit serum.