Figure 3.

EGFR is delivered to lysosomes upon mTOR inhibition in GBM cells. U87MG treated with Torin1 for 24h or untreated (CTR) were subjected to immunocytochemistry and confocal analysis for EGFR (red) and for different subcellular markers (green): Calnexin for endoplasmic reticulum (A), Giantin for Golgi cisternae (B), Tomm-20 for mitochondria (C) and Cathepsin D for lysosomes (D). The images showing the merge of the two signals are shown in the right panels. Inset containing a high magnification view of the merge image is also shown. Scale bar, 30 μM. Colocalisation was assessed by calculating the Pearson’s correlation coefficient r (mean r in D: CTR, 0.2 ± 0.03; Torin1, 0.75 ± 0.06).