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. 2020 Aug 13;12(8):2266. doi: 10.3390/cancers12082266

Figure 5.

Figure 5

The mitogen activated protein (MAPK)/ERK pathway is down-regulated upon mTOR inhibition in GBM cells. (A,B) U87MG, GL15 were cultured in complete DMEM medium in the presence (TORIN1) or absence (CTR) of 250 nM Torin1 and analysed at 2 h and 4 h (A) and at 24 h and 48 h (B). Western blot analysis was performed by using specific antibodies for ERK1/2 and P-ERK1/2. HSP90 was used as loading control. (C) GH2 cells were cultured in complete DMEM medium in the presence (TORIN1) or absence (CTR) of 250 nM Torin1 and analysed at the indicated time points. Western blot analysis was performed by using specific antibodies for ERK1/2 and P-ERK1/2. HSP90 was used as loading control. The graph represents the mean ± SD of three different experiments. Statistical significance: * p < 0.05 Student t-test ** p < 0.01 Student t-test, *** p < 0.001 Student t-test.