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. 2020 Aug 9;9(8):1864. doi: 10.3390/cells9081864

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Left: Concentration of γ-H2AX was determined with ELISA. Right: Representative immunofluorescence staining images of γ-H2AX intensity following SAM treatment were captured. (b) Left: Micronucleus scoring was performed on the slides stained with DAPI and anti-γ-H2AX (* p ≤ 0.05). Right: Arrows indicate representative micronuclei with γ-H2AX positivity. (c) Left: Percentage of DNA in the tail was referred as comet tail DNA % (* p ≤ 0.05). Right: Characteristic comets are visualized following SAM treatment.