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. 2020 Aug 4;9(8):1834. doi: 10.3390/cells9081834

Figure 2.

Figure 2

Assembly of GFP-NE81-S122EΔNLSΔCLIM is wavelength-dependent and occurs only upon blue, but not green or near UV light exposure. (A,B) Selected time points of live cell confocal spinning disk microscopy series comparing response to green light (561 nm) (A) or near UV light (405 nm) (B) versus blue light (488 nm). The same representative cell is shown in (A–A’’) and (B–B’’), respectively; the left cell shows the situation at t = 0 (recording of one image stack at 8% AOTF transmission). Laser excitations to trigger assembly in (A’,A’’,B’,B’’) were for 3 min at 40% AOTF transmission. Maximum intensity projections of fifteen focal planes at a distance of 0.27 µm are shown. Scale bars = 5 µm.