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GFP-NE81-S122EΔNLSΔCLIM assembly is triggered by cell flattening. Confocal spinning disk live cell imaging in LoFlo medium: (A,A’) two control examples prior to agar overlay; and (B,B’) two examples with two cells each after cell flattening by agar overlay, (B) in an early phase up to 1 h and (B’) in a late phase (after 1 h). Scale bar = 5 μm.
