Figure 2.

IL-8 produced by the BEAS-2B cells in response to primo-colonizing Staphylococcus strains. All strains of Staphylococcus (EMSa) and Streptococcus (EMS) were isolated from the buccal cavity of human newborns. Staphylococcus was isolated on the first day of life. For the challenge assay, BEAS-2B cells were co-incubated with bacterial strains (MOI of 100:1) for 6 h in 24-well plates. IL-8 was quantified by using ELISA immunoassays. Data are the mean of secreted IL-8 (pg/mL) ± SEM of at least two biological replicates (with two technical replicates each). p-values: *: p < 0.03, **: p < 0.002, ***: p < 0.0002 and ****: p < 0.0001.