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. 2020 Jul 22;8(8):1094. doi: 10.3390/microorganisms8081094

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cytokines and chemokines profiling of the BEAS-2B cells in response to bacterial stimulation: (a) IL-8, (b) IL-6, (c) TNF-α, (d) CCL2, (e) CCL20 and (f) CXCL2. Strains are identified as follows: EMS, Streptococcus strains; EME, Enterococcus strains; EMP, Pantoea. For the challenge assay, BEAS-2B cells were co-incubated with bacterial strains (MOI of 100:1) for 6 h, in 25 cm² tissue culture flasks. Cytokines and chemokines were quantified by using multiplex-magnetic-beads-based assay. Data are the mean of secreted proteins (pg/mL) ± SEM of four biological replicates (with two technical replicates each). p-values: *: p < 0.03, **: p < 0.002, ***: p < 0.0002 and ****: p < 0.0001.

Cytokines and chemokines profiling of the BEAS-2B cells in response to bacterial stimulation: (a) IL-8, (b) IL-6, (c) TNF-α, (d) CCL2, (e) CCL20 and (f) CXCL2. Strains are identified as follows: EMS, Streptococcus strains; EME, Enterococcus strains; EMP, Pantoea. For the challenge assay, BEAS-2B cells were co-incubated with bacterial strains (MOI of 100:1) for 6 h, in 25 cm² tissue culture flasks. Cytokines and chemokines were quantified by using multiplex-magnetic-beads-based assay. Data are the mean of secreted proteins (pg/mL) ± SEM of four biological replicates (with two technical replicates each). p-values: *: p < 0.03, **: p < 0.002, ***: p < 0.0002 and ****: p < 0.0001.