Table 3.
Content of phenolic acids and flavonoids in the flowering aerial parts of Hemerocallis cultivars. Mean values of three tests with standard deviation (± SD). Abbreviations: H1—H. fulva var. kwanso; H2—H. “Aten”; H3—H. “Bożena”; H4—H. “Catherine Woodbuery”; H5—H. “Chicago Apache”; H6—H. “Danuta”; H7—H. “Jaskółka”; H8—H. “Rebel Cause”; nd—Not detected; LOQ—Limit of quantification; DE—Dry extract; RT—Retention time; CE—Collision energy.
| No | Compound | RT [min] | [M–H]−[m/z] | Fragment Ions [m/z] | CE [eV] | Amounts [µg/g DE] | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | ||||||
| 1 | gallic acid | 5.07 | 168.7 | 78.9 124.9 |
−36 −14 |
<LOQ | <LOQ | nd | nd | <LOQ | <LOQ | nd | <LOQ |
| 2 | myricetin | 5.95 | 316.7 | 136.9 150.9 |
−32 −26 |
nd | <LOQ | nd | nd | nd | <LOQ | nd | nd |
| 3 | kaempferol | 7.56 | 284.7 | 116.8 93.0 |
−46 −52 |
nd | nd | nd | nd | <LOQ | <LOQ | nd | nd |
| 4 | protocatechuic acid | 8.23 | 152.9 | 80.9 107.8 |
−26 −38 |
45.9 ± 0.6 | 74.5 ± 2.8 | 86.4 ± 1.1 | 63.2 ± 0.2 | 94.0 ± 2.0 | 116.8 ± 0.5 | 61.5 ± 1.5 | 577.5 ± 12.5 |
| 5 | chlorogenic acid | 9.30 | 352.9 | 190.8 84.9 |
−24 −60 |
982.5 ± 5.0 | 1010.0 ± 10.0 | 461.3 ± 11.3 | 356.3 ± 6.3 | 986.3 ± 8.8 | 209.8 ± 1.0 | 945.0 ± 5.0 | 291.3 ± 1.3 |
| 6 | cis-sinapic acid | 9.78 | 22.8 | 121.0 148.9 |
−36 −20 |
<LOQ | <LOQ | <LOQ | nd | nd | <LOQ | nd | nd |
| 7 | rosmarinic acid | 10.23 | 358.7 | 132.6 160.8 |
−44 −20 |
nd | 12.0 ± 0.2 | nd | nd | nd | nd | nd | nd |
| 8 | 4-hydroxybenzoic acid | 11.27 | 136.8 | 92.9 107.9 |
−18 −18 |
<LOQ | 20.6 ± 0.3 | 9.6 ± 0.2 | 46.8 ± 0.4 | 5.1 ± 0.1 | 373.8 ± 1.3 | <LOQ | <LOQ |
| 9 | syringic acid | 11.41 | 196.9 | 122.8 181.9 |
−24 −12 |
<LOQ | 4.9 ± 0.2 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ |
| 10 | cis-caffeic acid | 11.68 | 178.7 | 88.9 134.9 |
−46 −16 |
45.4 ± 1.1 | 24.4 ± 0.9 | 13.3 ± 0.4 | 10.0 ± 0.1 | 21.2 ± 0.1 | 72.4 ± 1.9 | 10.8 ± 0.3 | 98.0 ± 1.0 |
| 11 | quercetin-3-O-rutinoside (rutin) | 11.99 | 608.7 | 299.6 270.9 |
−46 −60 |
927.9 ± 22.9 | 213.6 ± 5.3 | 251.6 ± 6.6 | 139.0 ± 2.5 | 1403.2 ± 43.7 | 114.7 ± 0.3 | 1049.7 ± 32.8 | 223.4 ± 1.9 |
| 12 | quercetin-3-O-galactoside (hyperoside) | 12.80 | 462.7 | 299.7 254.7 |
−28 −42 |
<LOQ | 193.8 ± 2.2 | 214.7 ± 5.9 | 128.8 ± 3.9 | <LOQ | 319.6 ± 12.7 | <LOQ | <LOQ |
| 13 | quercetin-3-O-glucoside (isoquercetin) | 13.00 | 462.7 | 299.7 270.7 |
−30 −44 |
1727.9 ± 13.9 | <LOQ | <LOQ | <LOQ | 366.1 ± 2.1 | <LOQ | 1699.4 ± 51.6 | 238.8 ± 2.8 |
| 14 | kaempferol-3-O-rutinoside (nicotiflorin) | 13.31 | 592.7 | 284.8 226.7 |
−38 −68 |
183.4 ± 3.8 | <LOQ | 17.2 ± 0.4 | 127.2 ± 2.3 | 269.4 ± 9.6 | <LOQ | 105.5 ± 2.4 | <LOQ |
| 15 | isorhamnetin-3-O-rutinoside (narcissoside) | 13.52 | 622.8 | 314.9 298.8 |
−40 −52 |
151.4 ± 85.0 | 15.1 ± 0.1 | 29.7 ± 1.0 | 48.4 ± 0.6 | 1160.0 ± 19.5 | 49.7 ± 0.7 | 57.6 ± 2.1 | <LOQ |
| 16 | p-coumaric acid | 14.28 | 162.8 | 93.0 119.0 |
−44 −14 |
34.6 ± 0.4 | 53.1 ± 0.9 | 13.1 ± 0.2 | 11.8 ± 0.0 | 20.1 ± 0.6 | 65.0 ± 0.8 | 36.9 ± 1.2 | 19.1 ± 0.3 |
| 17 | kaempferol-3-O-glucoside (astragalin) | 14.66 | 446.7 | 226.8 254.8 |
−54 −40 |
<LOQ | <LOQ | <LOQ | <LOQ | <LOQ | nd | <LOQ | <LOQ |
| 18 | isorhamnetin-3-O-glucoside | 14.76 | 476.8 | 313.9 270.9 |
−30 −44 |
<LOQ | <LOQ | <LOQ | <LOQ | 245.1 ± 1.2 | <LOQ | <LOQ | <LOQ |
| 19 | quercetin 3-O-rhamnoside (quercitrin) | 14.83 | 446.7 | 299.7 270.7 |
−30 −40 |
76.6 ± 1.9 | 40.0 ± 0.3 | 51.3 ± 1.2 | <LOQ | 238.5 ± 7.7 | 376.3 ± 0.0 | 81.3 ± 1.2 | 123.9 ± 4.2 |
| 20 | o-coumaric acid | 17.17 | 162.8 | 119.0 93.0 |
−14 −46 |
<LOQ | nd | nd | nd | nd | nd | nd | nd |
| 21 | quercetin | 17.91 | 300.7 | 150.9 178.8 |
−26 −20 |
10.7 ± 0.1 | <LOQ | <LOQ | <LOQ | 12.1 ± 0.1 | 50.9 ± 1.1 | 1.5± 0.1 | 42.3 ± 1.2 |
| 22 | salicylic acid | 18.06 | 136.9 | 75.0 93.0 |
−48 −16 |
<LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ |
| 23 | isorhamnetin | 19.09 | 314.7 | 299.7 150.9 |
−20 −30 |
<LOQ | <LOQ | <LOQ | <LOQ | 0.3 ± 0.0 | 6.0 ± 0.1 | <LOQ | <LOQ |