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Supplementation of 8 mM alanyl-glutamine (AlaGln) to CPDF and LPDF increased electrical resistance of HUVEC after 1 and 5 h (A). 10 kDa and 70 kDa dextran transport was reduced with CPDF only (B). Reduction of ZO-1 and CLDN5 protein abundance in CPDF treated HUVEC was restored with AlaGln supplementation (C). Data are mean ± SD. A.U. = arbitrary units. * p < 0.05, ** p < 0.01.
