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. 2020 Aug 8;9(8):2575. doi: 10.3390/jcm9082575

Figure 2.

Figure 2

Measurements of Micro-vessel Architecture. Slide specimens labeled with anti-Collagen IV Ab were imaged with a 40× objective and deconvoluted with AutoQuant® software for reduced light scatter and improved micro-vessel delineation. For each micro-vessel (overall diameter ≤15μm), two perpendicular line profiles of Collagen IV fluorescence intensity were generated with a custom MatLab program. Gaussian distributions (red) were fitted to the raw fluorescence intensity data (blue). The thickness of the Collagen IV ring (basement membrane) for each micro-vessel was determined as the average distance at the 95% confidence interval for each of the Gaussian curves. The inner diameter of the basement membrane was determined as the mean of the distances between the pairs of Gaussian curves at the locations of their 95% confidence intervals.