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. 2020 Aug 8;9(8):2575. doi: 10.3390/jcm9082575

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dimensions of the architectural features of the micro-vessel basement membrane (BM) are highly coordinated across control subjects and PAD patients. BMs of calf muscle biopsy specimens were labeled with anti-Collagen IV antibody, and then micro-vessel BM architectural features, including thickness, inner diameter and overall diameter were measured. Measurements were obtained for approximately 50 to 200 micro-vessels per biopsy specimen of control subjects (N = 14) and patients with Stage II (N = 15) and Stage IV (N = 16) PAD. Means per patient are plotted. Correlations were determined by Spearman Rho.