Table 2.

Measurements of the basement membrane of micro-vessels in the calf muscle of control subjects and patients with Stage II and Stage IV peripheral artery disease (PAD).

Micro-Vessel BM Measurements (μm)	Control	Stage II PAD	Stage IV PAD
Thickness	1.408 ± 0.025	1.577 ± 0.030 *	1.747 ± 0.060 *,†
Inner Diameter	3.243 ± 0.094	3.295 ± 0.064	3.972 ± 0.124 *,ǂ
Overall Diameter	6.058 ± 0.111	6.448 ± 0.112	7.466 ± 0.224 *,ǂ

Calf muscle biopsy specimens were labeled with anti-Collagen IV antibody and micro-vessel BM architectural features were measured. Measurements were obtained for approximately 50 to 200 micro-vessels per biopsy specimen. Control (N = 14), PAD Stage II (N = 15), and PAD Stage IV (N = 16) measurements are presented as mean ± S.E.M. Data were analyzed by Student’s t-test. * Significantly different from Control (p < 0.001). ^† Significantly different from Stage II PAD (p = 0.02). ^‡ Significantly different from Stage II PAD (p < 0.001).