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. 2020 Jul 22;9(8):182. doi: 10.3390/biology9080182

Figure 4.

Figure 4

Application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of SARS-CoV-2. (A) Location of RdRp primer sequence in the SARS-CoV-2 genome. (B) Cross-reactivity test of the novel SARS-CoV-2 RT-LAMP assay to other common respiratory viruses. Viral RNA isolated from a COVID-19 positive patient was used as a positive control (PC). NTC, non-template control. (C) RT-LAMP based colorimetric visual detection of SARS-CoV-2 RNA standards. (D) Clinical samples from positive (+) or negative (−) COVID-19 cases. NTC: non-template control. (E) Schematic representation of the SARS-CoV-2 DETECTR method workflow such as RNA extraction. The DETECTR method that involves the LAMP preamplification, and Cas12-based gene detection, and visualization by a fluorescent reader or lateral flow strip (TwistDx). (F) LoD for DETECTR assay showing the fluorescence values using SARS-CoV-2 DETECTR assay (n = 6) using SARS-CoV-2 N2 gene in vitro-transcribed (IVT) RNA. Representative lateral flow results for the assay of samples with 0 copies per μL and 10 copies per μL viral RNA. (G) Lateral flow strips showing SARS-CoV-2 DETECTR assay results after 3 min of flow. A–D are reproduced with permission from Lu et al. [70]. E–G are reproduced with permission from Broughton et al. [83].