Figure 5.
HSP70–eIF4G interaction promotes cellular protein synthesis. (A,B) Cells were incubated with 10 µM CoCl2 for 12 h; each group was then examined via protein synthesis assay. Representative pictures of protein synthesis are shown. Magnification 20×; Scale bar 50 μm. (C,D) Cells were cultivated with 10 µM CoCl2 for 12 h and the eIF4G-eIF4E interaction examined in situ for each group. Representative images of the PLA assay are presented. Magnification 20×; Scale bar 50 μm. Quantitative data from three experiments with similar results were analyzed. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with corresponding control groups.