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. 2020 Aug 8;12(8):747. doi: 10.3390/pharmaceutics12080747

Figure 3.

Figure 3

Effect of lysosomal pH on quinacrine and EFV uptake in lysosome-enriched fractions. (A) The uptake of quinacrine (5 μM) in the fraction of TR-iBRB2 cells was examined in the presence or absence of 100 nM bafilomycin A1 at 37 °C for 30 min. (B) The uptake of EFV (1 μM) in the fraction of TR-iBRB2 cells was examined in the presence or absence of 100 nM bafilomycin A1 at 37 °C for 30 min. (C) The uptake of EFV (1 μM) in the fraction of RPE-J cells was examined in the presence or absence of 100 nM bafilomycin A1 at 37 °C for 30 min. Bafilomycin A1 is an inhibitor of V-ATPase that attenuate the lysosomal acidic interior pH, and the uptake was examined in the presence or absence of 100 nM bafilomycin A1 at 37 °C for 30 min. In a pretreat condition, fractions were treated with 100 nM bafilomycin A1, and the uptake was performed in the absence of bafilomycin A1. In an acute condition, fractions were treated with ICF-buffer, and the uptake was performed in the presence of 100 nM bafilomycin A1. Each column represents the mean ± S.E.M (n = 4). ** p < 0.01, significantly different from the control.