Figure 4.

Sequence chromatograms of CD32 third exon of a representative Holstein sire compared to an allele model based on DNA-Seq. DNA extracted from semen was used for simultaneous PCR amplification of the third exons encoding CD32. Chromatograms show five variations in which the ratio of nucleotide heights provided information on the number of encoding paralogs. Below the chromatogram, dots indicate similarity to the consensus sequence of 10 allele variants predicted by the assembled sequences and counts of this sire’s DNA-Seq reads. Putative amino acid translation is given below the consensus sequence, in which codons are annotated by alternating font and background color. Nucleotide and amino acid variations are highlighted in yellow. Arrowheads point to the two cysteines predicted to form the disulfide bond that stabilizes an extracellular structure of the immunoglobulin-like C-2 type.