Table 3.
Minimum Inhibitory Concentration/Minimum Cidal Concentration (µg mL−1) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Gram Positive | Gram Negative | |||||||||
Rods | Cocci | Rods | Cocci | |||||||
Compound | Bacillus Cereus | Listeria Monocytogenes | Corynebacterium Diphtheria | MR-Staphylococcus Aureus | Streptococcus Pyogenes | Escherichia Coli | Salmonella Enteritidis | Klebsiella Oxytoca | Neisseria Meningitides | Haemophilus Influenza |
1 | 4/4 | 8/8 | 16/32 | 2/4 | 1/2 | 32/128 | 64/128 | 128/>512 | 32/32 | 32/32 |
2 | 64/128 | 128/256 | 32/64 | 32/32 | 64/64 | NI | NI | NI | NI | NI |
3 | 2/16 | 4/16 | 16/64 | 2/4 | 1/16 | 32/128 | 2/4 | 16/32 | 4/16 | 1/2 |
Kanamycin | 32/32 | 64/64 | 128/NI | 128/128 | 256/NI | 4/4 | 8/16 | 4/16 | 16/16 | 32/32 |
Erythromycin | <1/2 | 4/4 | NI | 2/2 | 32/64 | 256/256 | NI | NI | NI | 128/128 |
NI; No inhibition observed at the concentrations tested (512–1 µg mL−1). Antibacterial activity was determined using the broth microdilution assay to determine the minimum inhibitory concentration (MIC) of the test compounds. Minimum bactericidal concentration (MBC) was assessed by plating samples from wells with negative growth in the MIC determination assay. Values represent the average MIC/MBC of three replicates (n = 3). In each case no deviation was observed and therefore the SD = ± 0.0. Kanamycin was used as a Gram-negative positive control and Erythromycin was used as a Gram-positive positive control in this study.