Figure 5.

E2F2 protein expression under miR-302b plus cisplatin treatment in an in vitro model and in silico E2F2 correlation with the YY1/ITGA6 biological axis. E2F2 expression after (A) si-E2F1 knockdown and (B) miR-302b transfection in MDA-MB-231 cells treated or not with cisplatin, respectively. Vinculin and β-actin were used as housekeeping controls. Data are representative of three independent experiments performed at least in triplicate. (C) Schematic representation of robust neighbor binding sites of YY1 and E2F2 on the ITGA6 promoter. Right panel shows TFs motif from the JASPAR database used in the analysis. (D) Correlation network of YY1 and human TFs predicted to physical interact with YY1.