Figure 4.

Quercetin and/or resveratrol modulate the expression of key PCa-related genes. The expression profiles of 84 genes involved in 12 different PCa-related phenomena were assessed using a Qiagen PCa RT² Profiler PCR array. (a) Heat maps of the gene expression in quercetin (Q), resveratrol (R), and quercetin–resveratrol (QR) treated tumors are represented as the fold change compared to the control tumors. The assay was performed using two biological pools (as described in materials and methods) in triplicate within each group. Increased levels are indicated by red and decreased levels are green. (b) Validation of key PCa genes identified from the PCa PCR array was performed by RT-qPCR analysis. Data are presented as the mean ± SEM of two biological pools of three animals per group (n = 6) in technical triplicate. A one-way ANOVA with Tukey’s multiple comparison test was performed using Graphpad Prism 5 software (* p < 0.05, ** p < 0.01, *** p < 0.001).