Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 7;12(8):2210. doi: 10.3390/cancers12082210

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Knockdown of FBXL8 suppressed the production of inflammatory cytokines, chemokines and growth factors in breast carcinoma cells. Culture supernatants collected from cells treated with FBXL8-specific RNAi for 48 h, were analyzed for secreted tumorigenic factors using human cytokine array and ELISA. (A) Compared to control siRNA, treatment of FBXL8-specific RNAi significantly reduced the production of cancer-promoting factors, including MCP-1, I-TAC, TECK, CTACK, MIF, GM-CSF, NT-3, FGF-6, angiogenin, EGFR, ICAM-1, Fas, DtK and TRALIR3. In contrast, FBXL8 knockdown elevated the secretion of pro-tumorigenic IL-6R and TNFR1 in BRCA. Osteoprotegerin (OPG) shows opposite trend in MCF7 and MDA-MB231 cells. Blue boxes: down-regulation and red boxes: up-regulation of factors secreted by FBXL8-knockdowned cells. The numbers next to the blue/red boxes indicate: (1) Angiogenin, (2) FGF-6, (3) GM-CSF, (4) MCP-1, (5) NT-3, (6) CTACK, (7) DtK, (8) EGFR, (9) Fas, (10) ICAM-1, (11) IL-6R, (12) I-TAC, (13) MIF, (14) OPG, (15) TNFR1, (16) TECK and (17) TRALIR3. The array membranes are spotted in duplicate. (B) ELISA confirmed differential quantities of GM-CSF, MCP-1, CTACK, EGFR, ICAM-1, I-TAC, MIF and TECK secreted from MCF7 and MDA-MB231 cells when FBXL8 was knocked down (blue bars). Data are representative of means ± SD (n = 3). **, p < 0.01, ***, p < 0.001.

Knockdown of FBXL8 suppressed the production of inflammatory cytokines, chemokines and growth factors in breast carcinoma cells. Culture supernatants collected from cells treated with FBXL8-specific RNAi for 48 h, were analyzed for secreted tumorigenic factors using human cytokine array and ELISA. (A) Compared to control siRNA, treatment of FBXL8-specific RNAi significantly reduced the production of cancer-promoting factors, including MCP-1, I-TAC, TECK, CTACK, MIF, GM-CSF, NT-3, FGF-6, angiogenin, EGFR, ICAM-1, Fas, DtK and TRALIR3. In contrast, FBXL8 knockdown elevated the secretion of pro-tumorigenic IL-6R and TNFR1 in BRCA. Osteoprotegerin (OPG) shows opposite trend in MCF7 and MDA-MB231 cells. Blue boxes: down-regulation and red boxes: up-regulation of factors secreted by FBXL8-knockdowned cells. The numbers next to the blue/red boxes indicate: (1) Angiogenin, (2) FGF-6, (3) GM-CSF, (4) MCP-1, (5) NT-3, (6) CTACK, (7) DtK, (8) EGFR, (9) Fas, (10) ICAM-1, (11) IL-6R, (12) I-TAC, (13) MIF, (14) OPG, (15) TNFR1, (16) TECK and (17) TRALIR3. The array membranes are spotted in duplicate. (B) ELISA confirmed differential quantities of GM-CSF, MCP-1, CTACK, EGFR, ICAM-1, I-TAC, MIF and TECK secreted from MCF7 and MDA-MB231 cells when FBXL8 was knocked down (blue bars). Data are representative of means ± SD (n = 3). **, p < 0.01, ***, p < 0.001.