Figure 1.

Histological characterization of the decellularized scaffolds repopulated with adult renal stem-like PKH^high/CD133+/CD24− cells (RSC). (a) Representative Z-stack 3D reconstruction of images after DAPI (blue) and phalloidin (purple) immunofluorescence staining of the scaffolds at 30 days after RSC seeding. Total thicknesses of the shown structure was 31.2-µm original magnification, ×400; (b–c) five independent experiments of human kidney scaffold repopulation for 30 days with RSC in presence of basal medium were performed; (b) representative Hematoxylin and Eosin (H&E) staining of formalin fixed, paraffin embedded (FFPE) repopulated scaffold sections. Scale bars, 100 µm; (c) representative sequential IF analysis of the FFPE-repopulated scaffolds with the antibodies against the indicated markers that recognize specific tubular or vascular phenotypes. The different antibodies combinations identify proximal tubules (top), distal tubules (middle) and endothelium (bottom). Scale bars, 50 µm. CK—Cytokeratin; AQP—Aquaporin; vWf—Von Willebrand Factor; blue—DAPI.